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实验小鼠

组织样品检测

组织样品检测
  • 胸腺:研究方案T上皮细胞分解、发育期;
  • 淋巴腺结:T癌生殖细胞系、B癌生殖细胞系、DC癌生殖细胞系研究分析;
  • 骨髓:B生殖体细胞膜胸部骨骼发育调查,红生殖体细胞膜胸部骨骼发育调查,造血干生殖体细胞膜调查;
  • 肾聚集:分折炎性生殖细胞浸泡;
  • 肺组建开展:小鼠肺组建开展中免疫系统性机构组合成深入定性分析,肺部肿瘤或感染小鼠肺组建开展中免疫系统性机构浸润性深入定性分析;
  • 皮:細胞炎性浸润性具体分析;
  • 脑企业:深入分析企业中炎性组织细胞侵及前提。


检测例案一:胸腺T组织细胞发展


Population

Gating Step

Th Cells

Live single

mCD4+

mCD8-

 /

 /

Tc Cells

Live single

mCD4-

mCD8+

 /

 /

DP Cells

Live single

mCD4+

mCD8+

 /

/

DN Cells

Live single

mCD4-

mCD8-

 /

/

DN2 Cells

Live single

mCD4-

mCD8-

mCD44+

mCD25+

DN3 Cells

Live single

mCD4-

mCD8-

mCD44-

mCD25+

DN1 Cells

Live single

mCD4-

mCD8-

mCD44+

mCD25-

DN4 Cells

Live single

mCD4-

mCD8-

mCD44-

mCD25-


图片40.png

科学试验案例分享二:肾脏免疫系统肿瘤细胞分折(12色解决方案)

Population

Gating Step

Total Leukocytes

Live single

mCD45+

/

/

/

Neutrophiles

Live single

mCD45+

mCD11b+

Ly6G+

/

Monocytes

Live single

Not Neutrophiles

mCD11b+

Ly6C hi

/

Eosinophils

Live single

Not monocytes

mCD11b+

SSC-H hi

/

Macrophage

Live single

Not eosinophils

mCD11b+

F4/80+

/

DC Cells

Live single

Not eosinophils

mCD11b+

mCD11c+

/

T Cells

Live single

Not eosinophils

mCD3+

mCD335-

/

B Cells

Live single

Not eosinophils

mCD19+

/

/

NK Cells

Live single

Not eosinophils

mCD3-

mCD335+

/

Th Cells

Live single

Not eosinophils

mCD3+

mCD4+

mCD8-

Tc Cells

Live single

Not eosinophils

mCD3+

mCD4-

mCD8+

图片41.png

實驗真实案例三:脑团队侵润免役癌细胞讲解(10色方法)


Population

Gating step

Total Leukocytes

Live single

mCD45+

/

/

/

Neutrophiles

Live single

mCD45+

mCD11b+

Ly6G+

/

Monocytes

Live single

Not Neutrophiles

mCD11b+

Ly6C hi

/

Eosinophils

Live single

Not monocytes

mCD11b+

SSC-H hi

/

Macrophage

Live single

Not eosinophils

mCD11b+

F4/80+

/

T cells

Live single

Not eosinophils

mCD3+

mCD19-

/

B cells

Live single

Not eosinophils

mCD19+

mCD3-

/

Th

Live single

Not eosinophils

mCD3+

mCD4+

mCD8-

Tc

Live single

Not eosinophils

mCD3+

mCD4-

mCD8+


图片42.png

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